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GenomeTag™ clones are full-length cloned human or mouse genes that carry N- or C-terminal GFP, luciferase or epitope tags. The cloned genes are based on end sequenced fosmid clones, rather than cDNA. Each tagged clone carries the complete gene of interest embedded in approximately 40 kb of native human or mouse genomic DNA, with the reporter sequence (Renilla mulleri GFP, Gaussia princeps luciferase or epitope tag) fused to the extreme 5'- or 3'-end of the protein-coding sequence. The clones include genomic sequences required for natural regulation of transcription, including introns and thousands of nucleotides upstream and downstream of the transcription unit. Clones are transfection ready and are available with a neomycin or puromycin resistance gene in the fosmid backbone to facilitate production of stable transfectants. Choice of GFP, luciferase or epitope tags at N- or C-terminus is available. Click here for the list of tagged genes currently available. Please call if your gene of interest is not on the list.

Conventional cDNA Clones

GenomeTag Clones
 

Key features of GenomeTag clones:

• Tagged proteins are produced at physiologically correct times and levels because genomic sequences required for natural regulation of transcription, including introns and thousands of nucleotides upstream and downstream of the transcription unit are present.

• Tagged proteins have an optimal probability of exhibiting native subcellular localization and native biological function. Proteins carrying reporter tags at their termini have a high likelihood of exhibiting native biological function. Also, because they are expressed under the same regulatory controls as their untagged counterparts, they generally exhibit appropriate subcellular localization and do not perturb the natural physiology of the cell.

• All physiologically appropriate splicing isoforms are produced because all native introns and exons are present in the tagged genes.

Full-length tagged genomic clones are useful for studies and assay development involving:

• Transcriptional and translational regulation

• Post-translational modification

• Protein subcellular localization

• Protein-protein interactions

• Alternative splicing

• Knockdown of RNA/protein by siRNA/miRNA

• Gene replacement following knockout

• Transgenic production

GFP-Mortalin (mitochondria)	GFP-Hmga 1 (chromatin)	GFP-Calmodulin (stress fibers)
Pictures courtesy J.Jarvik, Carnegie Mellon University

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