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About Nanoclusters
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Technology
ProteoColor™
Trilite™
Nanoclusters are aggregates of individual quantum dots. Unlike standard quantum dots that are size-tunable
(i.e. the emission wavelength is determined by the crystal size)
those used for ProteoColor Trilite Nanoclusters are composition-tunable;
quantum dot colors are tuned by manipulating semiconductor
materials in the core.
As a result, all ProteoColor Trilite Nanoclusters have the same average
diameter (25 nm), mass, photostability and quantum efficiency,
irrespective of emission wavelength. Further, the excitation
spectra are broad. Any wavelength below the emission wavelength
can be used for excitation. This means that a single wavelength
in the ultraviolet spectrum can excite all 10 Trilite Nanoclusters. The
core/shell of the Trilite Nanoclusters contains CdSxSe1-x/ZnS.
ProteoColor Trilite Nanoclusters can be used for a wide range of life
science applications including Western blot detection, cell
tracing and immunohistochemistry. They are available
functionalized with carboxyl groups to which biological
molecules can be conjugated. Alternatively, Trilite Nanoclusters
conjugated to avidin are available for use with biotin-tagged
reagents.
ProteoColor Trilite Nanoclusters are available in the 10
standard colors shown below.
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ProteoColor |
Wavelength (nm) |
|
| |
Violet |
420 |
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Blue |
490 |
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| |
Aqua |
510 |
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| |
Grass Green |
525 |
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| |
Deep Green |
540 |
|
| |
Yellow |
575 |
|
| |
Orange |
600 |
|
| |
Scarlett |
630 |
|
| |
Red |
665 |
|
| |
Dark Red |
685 |
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Key features of ProteoColor Trilite Nanoclusters:
- Trilite Nanoclusters are brighter than individual
quantum dots (Figure 1).
- They have narrow emission wavelengths,
making it easier to discern different colors (Figure 2).
- They have superior photostability and show
no photobleaching. Trilite Nanoclusters of all colors are equally photostable.
- Trilite Nanoclusters of all colors have similar
size and mass.
- Quantum efficiencies are similar for all
colors. Thus all colors are equally bright.
- A single wavelength of ultraviolet light
can be used to excite all colors of Trilite Nanoclusters.

Figure 1:
Individual particles of functionalized ProteoColor Trilite
Nanoclusters are brighter than individual functionalized
nanocrystals.

Figure
2: Emission spectra of the 10 standard colors.
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